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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 5137386, 8 pages
Research Article

Polysaccharide Extracted from Laminaria japonica Delays Intrinsic Skin Aging in Mice

1Department of Physiology, Guangxi Medical University, Nanning 530021, China
2Center of Translational Medicine, Guangxi Medical University, Nanning 530021, China
3First Clinical Medical College, Guangzhou Medical University, Guangzhou 511436, China
4Eighth Affiliated Hospital, Guangxi Medical University, Guigang 530007, China

Received 3 January 2016; Revised 6 March 2016; Accepted 9 March 2016

Academic Editor: Yong C. Boo

Copyright © 2016 Longyuan Hu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study aimed to determine the effect of topically applied Laminaria polysaccharide (LP) on skin aging. We applied ointment containing LP (10, 25, and 50 μg/g) or vitamin E (10 μg/g) to the dorsal skin of aging mice for 12 months and young control mice for 4 weeks. Electron microscopy analysis of skin samples revealed that LP increased dermal thickness and skin collagen content. Tissue inhibitor of metalloprotease- (TIMP-) 1 expression was upregulated while that of matrix metalloproteinase- (MMP-) 1 was downregulated in skin tissue of LP-treated as compared to untreated aging mice. Additionally, phosphorylation of c-Jun N-terminal kinase (JNK) and p38 was higher in aging skin than in young skin, while LP treatment suppressed phospho-JNK expression. LP application also enhanced the expression of antioxidative enzymes in skin tissue, causing a decrease in malondialdehyde levels and increases in superoxide dismutase, catalase, and glutathione peroxidase levels relative to those in untreated aging mice. These results indicate that LP inhibits MMP-1 expression by preventing oxidative stress and JNK phosphorylation, thereby delaying skin collagen breakdown during aging.