Calycosin and Formononetin Induce Endothelium-Dependent Vasodilation by the Activation of Large-Conductance Ca2+-Activated K+ Channels (BKCa)
Formononetin increased outward currents in HUVEC through channel. (a) graph in response to formononetin (1–100 μM). (b) Dose-response curve for whole cell recording of currents at +100 mV with different concentrations of formononetin (10 nM–100 μM). (c, d) Representative trace of currents that were recorded in response to formononetin, in the absence and presence of (c) TEACl (1 mM) or (d) IbTX (200 nM). (e, f) Whole cell recording of currents at +100 mV in response to formononetin (100 μM) in the presence of (e) apamin (200 nM), IbTX (200 nM), ChTX (200 nM), or TEACl (1 mM; versus control; and versus formononetin-treated cells), or (f) with different as indicated ( versus formononetin-treated cells with free ). Data were shown as mean ± SEM. ChTX, charybdotoxin; IbTX, iberiotoxin; TEACl, tetraethylammonium chloride.