Protective Effects of Panax notoginseng Saponins against High Glucose-Induced Oxidative Injury in Rat Retinal Capillary Endothelial Cells

Figure 3

PNS inhibited the ROS and MDA induced by high glucose in rat RCECs. Seeded in plates or flasks, rat RCECs were incubated with PNS (100 μg/mL) in 30 mM glucose for 72 h. Fluorogenic probes (DCFH-DA, DHE, and MitoTracker Red CM-H₂XRos) showed that, after 72 h of PNS treatment, the intracellular and and mitochondrial ROS were decreased (a). A lipid peroxidation assay showed that PNS could decrease the MDA content (b). Data are expressed as the mean ± SD (). versus 5.5 mM glucose; versus 30 mM glucose. Scale bar: 33 μm.

(a)

(b)