Effects of Cymbidium Root Ethanol Extract on Atopic Dermatitis

Figure 3

Effect of Cymbidium ethanol extract (CYM) on 2,4-dinitrochlorobenzene- (DNCB-) induced histopathological changes and immunoglobulin E (IgE) level in atopic dermatitis- (AD-) like mouse model. After inducing AD, 10 mg/mL CYM solution (in 3 : 1 mixture of acetone and olive oil) was applied to the dorsal skin of mice for a total of 6 times over a 2-week period. (a) After euthanasia, dorsal skin lesions were enucleated from mice in all three groups (non-, DNCB, DNCB, and CYM treatment), fixed, cryoprotected, sectioned, and stained with hematoxylin and eosin (H&E). (b) Densitometric analysis of epidermal thickness of each group was performed. To measure thickness of tissues, densitometric analysis was performed using a Nikon light microscope and an associated program. Data are mean ± standard deviation (SD, ); versus DNCB treatment. (c) Serum IgE was analyzed using a mouse IgE kit and was determined relative to standard absorbance values measured at 450 nm using a microplate reader. Data are mean ± SD (); versus DNCB treatment.

(a)

(b)

(c)