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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 5698685, 8 pages
Research Article

In Vitro Antiproliferative and Antioxidant Effects of Extracts from Rubus caesius Leaves and Their Quality Evaluation

1Department of Pharmacognosy, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland
2Department of Virology and Immunology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland
3Department of General Ophthalmology, Medical University of Lublin, ul. Chmielna 1, 20-079 Lublin, Poland
4Department of Industrial Microbiology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland
5Department of Organic Chemistry, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok, Poland
6Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland

Received 18 July 2016; Accepted 6 December 2016

Academic Editor: Dolores García Giménez

Copyright © 2016 Daniel Mirosław Grochowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study was performed to evaluate the effect of different extracts and subfractions from Rubus caesius leaves on two human colon cancer cell lines obtained from two stages of the disease progression lines HT29 and SW948. Tested samples inhibited the viability of cells, both HT29 and SW948 lines, in a concentration-dependent manner. The most active was the ethyl acetate fraction which, applied at the highest concentration (250 μg/mL), decreased the viability of cells (HT29 and SW948) below 66%. The extracts and subfractions were also investigated for antioxidant activities on DPPH and FRAP assays. All extracts, with the exception of water extract at a dose of 250 μg/mL, almost totally reduced DPPH. The highest Fe3+ ion reduction was shown for the diethyl and ethyl acetate fractions. It was more than 6.5 times higher (at a dose 250 μg/mL) as compared to the control. The LC-MS studies of the analysed preparations showed that all samples contain a wide variety of polyphenolics, among which ellagitannins turned out to be the main constituents with dominant ellagic acid, sanguiin H-6, and flavonol derivatives.