Evidence-Based Complementary and Alternative Medicine

Research Article

In Vitro Antiproliferative and Antioxidant Effects of Extracts from Rubus caesius Leaves and Their Quality Evaluation

Table 1

MS and UV-Vis data of compounds detected in extracts and fractions prepared from leaves of R. caesius.


Number	Compound name	Retention time [min]	UV [nm]	[M − H]⁻	MS² ions	MS³ ions	[M + H]⁺	MS² ions	MS³ ions	NL detected [amu]

(1)	Gallic	3.3	265	169	125b	—	171	—	—	—
(2)	Unknown phenolic acid	3.4	229, 314	183	139b	—	185	—	—	—
(3)	Unknown compound	7.2	214, 260, 292	363	329, 325, 278, 153b	—	365	333b, 289, 269	—	—
(4)	Unknown ellagitannin	10.2	260	/783	481, 301b, 377, 311	—		785, 767, 483, 465, 303, 277b	—	—
(5)	Methyl	13.9	271	183	125b	—	185	153b	—	—
(6)	Galloyl-HHDP-glucose isomer	15.6	260	633	481, 463, 343, 301b, 275	301b, 275		482, 465b, 447, 321, 303, 277	—	152
(7)	Unknown ellagitannin	16.3	263	/783	481, 301b, 377, 311	—		785, 767, 483, 465, 303, 277b	—	—
(8)	Galloyl-HHDP-glucose isomer	16.8	261	633	481, 463, 343, 301b, 275	301b, 275		482, 465b, 447, 321, 303, 277	—	152
(9)	Unknown phenolic acid	18.3	237, 301sh, 321	179	135b	—	181	—	—	—
(10)	Unknown ellagitannin	23.4	260	459	—	—	461	—	—	—
(11)	p-Coumaric acid	27.2	247, 300sh, 325	325	314, 163b, 259	—	355	233, 161b, 135	—	—
(12)	Methyl	29.9	211, 277, 356	305	273b, 245	—	307	293, 275, 247b, 219	—	—
(13)	Unknown compound	30.2	217, 252, 274, 337	330	327, 287b, 269, 214	—	332	314, 289b, 227	—	—
(14)	Unknown ellagitannin	32.2	261	/1567	1265, 1103, 933, 633, 301b	—		—	—	—
(15)	Unknown phenolic acid	32.5	218, 310	279	272, 190, 163b	—	281	147b	—	—
(16)	Ellagic acid derivative	33.7	250, 358	331	287b	—	333	315, 289b, 272	—	—
(17)	Unknown ellagitannin	34.1	262	/1567	1265, 1103, 933, 633, 301b	—	—	—	—	—
(18)	Quercetin	34.4	255, 263sh, 351	623	321, 301b	—	625	479b, 449, 303	303b	146
(19)	Quercetin	35.1	256, 264sh, 351	609	429, 301b, 285	—	611	479b, 449, 303	461, 303b	132
(20)	Sanguniin	35.5	263	/1103	935, 633, 541, 469, 301, 169	—	—	—	—	—
(21)	Ellagic acid pentoside	36.3	251, 354	433	387, 301b, 161	257b	435	417, 303b, 219	285b	162
(22)	Ellagic	37.0	250, 353	301	273, 257b, 245, 187	—	303	295, 285b, 257, 207, 147	—	—
(23)	Sanguniin H-	37.4	260	/1870	—	—	—	—	—	—
(24)	Unknown compound	37.5	255, 350	431	269b	—	433	271b, 174	—	—
(25)	Kaempferol	38.1	265, 347	607	321, 285b, 257	—	609	463b, 447, 287, 307, 231	447, 287b	146
(26)	Quercetin 3-O-	38.8	255, 263sh, 351	477	431, 301b, 179, 151	273, 257, 179b, 151	479	303b	—	176
(27)	Kaempferol	39.3	265, 343	593	307, 285b	—	595	549, 465b, 353, 287	445, 329, 287b	132
(28)	Unknown compound	40.1	251, 324	309	277, 179, 161b	—	311	—	—	—
(29)	Unknown ellagitannin	42.3	260	/1117	935, 633b, 483, 459, 301	—
(30)	Kaempferol 3-O-	43.1	263, 341	461	415, 285b, 257, 175	267, 257b, 241, 229	463	445, 371, 287b, 203	258, 241b, 213, 173, 121	176
(31)	Unknown phenolic acid	45.8	230sh, 294sh, 328	359	249, 223, 197, 179, 161b	—	361	163b	—	—
(32)	Unknown phenolic acid	47.0	235, 298sh, 325	503	485, 341b, 281, 251, 221, 179	323, 281, 271b, 221, 179	504	—	—	162
(33)		58.5	266, 295sh, 315, 353sh	593	447, 307, 285b	327, 285b, 255	595	329, 309b, 287, 235, 217, 165	—	146
(34)	Quercetin derivative	59.9	255, 265sh, 353	533	515, 357, 301b	—	535	303b, 215	—	—
(35)	Kaempferol derivative	63.3	263, 343	663	645, 499, 399, 285b	—	665	519b, 379, 287	287b, 159	146

⁢, , , assignment, with chemical standard have been made, b: base peak (the most abundant ion in recorded spectrum), and in bold: ions subjected to MS³ fragmentation.