Activation of p53/miR-34a Tumor Suppressor Axis by Chinese Herbal Formula JP-1 in A549 Lung Adenocarcinoma Cells

Figure 5

JP-1 induces the transcription of miR-34a and decreases its downstream targets required for proliferation and apoptosis resistance in A549 cells. (a) RT-PCR analysis showed that the transcription of miR-34a was increased along with p53 protein induction (Western blot) after treatment with JP-1 for 48 h. (b) Western blot analysis showed the decreases of miR-34a downstream proteins, SIRT1, c-Myc, and survivin after treatment with JP-1 for 48 h. The U6 small nuclear RNA and GAPDH were used as the loading controls for RT-PCR and Western blot, respectively.

(a)

(b)