Activation of p53/miR-34a Tumor Suppressor Axis by Chinese Herbal Formula JP-1 in A549 Lung Adenocarcinoma Cells

Figure 6

JP-1 decreases the miR-34a downstream targets controlling EMT and inhibits the migration of A549 cells. (a) The decreases of miR-34a downstream EMT inducer (AXL) and marker (Snail) proteins after treatment with JP-1 for 48 h. The downregulation of another EMT marker Vimentin protein was also observed. Cell lysates were analyzed by Western blot, using GAPDH as the loading control. (b) Wound healing assay performed in IBIDI Culture-Inserts. A549 cells treated with JP-1 300 μg/mL or medium only (control) were seeded into different reservoirs of an IBIDI insert as described in Material and Methods. The closure of the gap was photographed by phase-contrast microscopy at 12, 24, and 48 h after treatment, scale bar = 50 μm. (c) The cell viability of A549 cells treated with JP-1 as that described for the wound healing assay. Data are expressed as mean ± SE and analyzed as described in Materials and Methods. At that cell density, JP-1 did not affect the cell viability of A549 cells within 48 h.

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