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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 6826175, 6 pages
Research Article

Effect of Alkaloids Isolated from Phyllodium pulchellum on Monoamine Levels and Monoamine Oxidase Activity in Rat Brain

1College of Pharmacy, Dalian Medical University, Dalian 116044, China
2Department of Neurosurgery, The Second Affiliated Hospital, Dalian Medical University, Dalian 116044, China
3Institute of Nationality Medicine in Guangxi, Nanning 530001, China

Received 30 September 2015; Revised 28 February 2016; Accepted 24 March 2016

Academic Editor: I-Min Liu

Copyright © 2016 Lu Cai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Phyllodium pulchellum (P. pulchellum) is a folk medicine with a significant number of bioactivities. The aim of this study was to investigate the effects displayed by alkaloids fractions, isolated from the roots of P. pulchellum, on neurotransmitters monoamine levels and on monoamine oxidase (MAO) activity. Six alkaloids, which had indolealkylamine or β-carboline skeleton, were obtained by chromatographic technologies and identified by spectroscopic methods such as NMR and MS. After treatment with alkaloids of P. pulchellum, the reduction of DA levels (54.55%) and 5-HT levels (35.01%) in rat brain was observed by HPLC-FLD. The effect of alkaloids on the monoamines metabolism was mainly related to MAO inhibition, characterized by IC50 values of and μg/mL for MAO-A and MAO-B, respectively. The acute toxicity indicated that P. pulchellum extract was nontoxic.