Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function

Figure 6

Impaired lymphatic thoracic duct formation induced by VEGFR-3 kinase inhibitor (MAZ51) was rescued by Du-Huo-Ji-Sheng-Tang in dose dependent manner. Two dpf zebrafish (fli1:egfp; gata1:DsRed) were treated with 30 μM MAZ51 for 6 hours and then changed to be treated with different doses of Du-Huo-Ji-Sheng-Tang (10–100 μg/mL) for 48 hours. Embryos treated with 0.2% DMSO served as a vehicle control. (a) Representative confocal images show that Du-Huo-Ji-Sheng-Tang increased lymphatic thoracic duct formation of zeberafish, white arrow indicates lymphatic thoracic duct, and white star indicates lack of lymphatic vessel. (b) Quantitation of the number of lymphatic thoracic ducts. (c) Quantitation of the length of lymphatic thoracic duct. Values are mean ± SD of 9–11 zebrafishes. versus MAZ51.

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