Bufalin Inhibits Proliferation and Induces Apoptosis in Osteosarcoma Cells by Downregulating MicroRNA-221

Figure 2

Bufalin downregulates miR-221 in U-2OS and Saos-2 cells. (a) Microarray analysis was used to compare the expression profiles of miRNAs in Saos-2 cells that were untreated or treated with bufalin at its IC50. miR-221, one of the most markedly downregulated miRNAs, was labeled with a red box. (b) Detected by qRT-PCR, miR-221 level dramatically decreased 2–5-fold after being treated with bufalin in both U-2OS and Saos-2 cells ( compared with the untreated control group).

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