Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum

Figure 1

Morphology and growth of mouse bone-marrow- (BM-) derived Mesenchymal Stem Cells (MSCs). (a) BM-MSCs were isolated based on adherence and cultured for 24 hours. (b) Primary MSCs cultured for 7 days; the cells showed colony-like growth, shuttle shape with synapses, large and clear nuclei, and abundant cytoplasm. (c) Primary MSCs cultured for 15 days; cells gradually became fused. (d) MSCs at first passage (P1) cultured after 7 days; cells diffraction was better. (e) MSCs at second passage (P2) cultured after 7 days. (f) MSCs at third generation (P3) cultured after 7 days; P1, P2, and P3 MSCs were all in homogeneous spindle shape growth (magnification: ×10). (g) BM-derived MSCs at P3 grew slowly at 1-2 days; cells were in a logarithmic growth phase at 3-4 days; and cells entered a platform phase after 7 days.

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