Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum

Table 1

Albumin (ALB) in second-passage (P2) bone marrow- (BM-) derived Mesenchymal Stem Cells (MSCs) determined by immunocytochemical staining (ICC).

	ALB^+ BM-derived MSCs (%)
	7 days	14 days	21 days	28 days
Negative controls	0	0	0	0
HGF + normal serum	0	24.96 ± 2.76	36.18 ± 1.45	67.15 ± 2.78^△
HGF + SDF-1 + normal serum	19.27 ± 1.91	47.22 ± 1.62	55.80 ± 1.03	71.03 ± 1.21^△
HGF + SDF-1 + YGJ	51.96 ± 3.17	65.53 ± 2.02	72.95 ± 0.95	76.84 ± 1.70
YGJ	8.06 ± 0.59	13.33 ± 1.75^#	21.43 ± 1.08^#	16.36 ± 2.68

Note. Negative controls: MSCs were cultured in medium containing 15% normal serum; HGF + normal serum: MSCs were cultured in medium supplemented with a final concentration of 20 ng/mL HGF and 15% normal serum; HGF + SDF-1 + normal serum: MSCs were cultured in medium supplemented with a final concentration of 20 ng/mL HGF + 50 ng/mL SDF-1 and 15% normal serum; HGF + SDF-1 + Yiguanjian drug serum: MSCs were cultured in medium supplemented with a final concentration of 20 ng/mL HGF + 50 ng/mL SDF-1 + 20% Yiguanjian drug serum. YGJ: MSCs were cultured in medium supplemented with 20% Yiguanjian drug-containing serum. Data are shown as mean ± standard deviation (SD) from 5 independent experiments. HGF + SDF-1 + Yinguanjian drug serum versus HGF + SDF-1 + normal serum at the same time. : 28 days versus 7 days under the same induction condition. : 14 days versus 7 days and 21 days versus 14 days under the same induction condition.