Asparagus cochinchinensis Extract Alleviates Metal Ion-Induced Gut Injury in Drosophila: An In Silico Analysis of Potential Active Constituents

Figure 2

A. cochinchinensis protects gut epithelial cells from metal ion-induced cell death. (a) The effect of AC extract on Cu-induced epithelial cell death. (b) The effect of AC extract on Zn-induced epithelial cell death. (c) The effect of AC extract on Al-induced epithelial cell death. ((d)–(f)) The comparison of gut necrotic cell quantity between control and AC group. The number of necrotic cells was quantified using ImageJ software (10–15 guts were examined to quantify necrotic cells for each group). value was calculated by Student’s -test. ; . Con: control, flies fed with metal ions without AC for 96 h; AC: flies fed with metal ions with A. cochinchinensis (AC) for 96 h. 7-AAD: 7-amino-actinomycin D, dead cells (red signal); DAPI: 4′,6-diamidino-2-phenylindole, nucleus (blue signal); 7-AAD/DAPI (red and blue signal). Scale bar: 100 μm.