Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion

Figure 5

EA increased the expression of the pCREB protein, BDNF protein, and miR132 and decreased the expression of p250GAP protein in the hippocampus of 2VO rats and these effects were partially inhibited by H89. (a) Immunoblot analysis for pCREB protein in hippocampus and histogram of its relative band intensity. (b) Immunoblot analysis of BDNF protein in hippocampus and histogram of its relative band intensity. (c) Histogram of fold change of miR132 in the hippocampus. (d) Immunoblot analysis for p250GAP protein in hippocampus and histogram of its relative band intensity. Quantitative analysis demonstrated that hippocampal pCREB, BDNF, and miR132 levels were decreased by 2VO and increased by EA treatment. Concurrently, EA reduced the elevation of p250GAP level in the hippocampus of 2VO rats. These protective effects were at least partially inhibited by H89 administration. Each value represents mean ± SEM, for each group. , compared with the control group; , compared with the model group; , compared with the model group; and , compared with the EA+NS group.

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