The Zuo Jin Wan Formula Induces Mitochondrial Apoptosis of Cisplatin-Resistant Gastric Cancer Cells via Cofilin-1

Figure 2

Effects of ZJW on mitochondrial apoptosis signalling pathways. (a) The levels of proteins in mitochondrial apoptosis signalling pathways were evaluated using western blotting in cisplatin (DDP)-resistant SGC7901/DDP cells following treatment with ZJW. Cytochrome C (C) represented the protein level of cytochrome C from cytoplasm. The results indicated dose- (treating time, 24 h) and time-dependent (ZJW, 50 μg/mL) effects. (b) After the same treating method as above, caspase-3 and caspase-9 activity were measured using a standard colorimetric assay (Bio-Rad). Relative caspase-3 and caspase-9 activity levels were normalized against control levels.

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