Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells

Table 1

Frequency of micronuclei (MN) and nuclear division index (NDI) obtained for V79 cell cultures treated with betulinic acid.

Treatment (μM)	MN frequency	NDI
Control	4.34 ± 0.58	1.69 ± 0.01
DMSO	3.67 ± 2.58	1.69 ± 0.03
2.7	5.34 ± 2.08	1.69 ± 0.01
5.5	5.67 ± 1.53	1.72 ± 0.01
11	4.67 ± 1.15	1.71 ± 0.02
22	7.67 ± 1.53	1.72 ± 0.02
MMS	42.34 ± 3.05	1.72 ± 0.02

Results are reported as mean ± standard deviation. DMSO: dimethyl sulfoxide (0.3 µM), MMS: methyl methanesulfonate (400 µM). A total of 3,000 binucleated cells were analyzed per treatment group. A total of 1,500 cells were analyzed per treatment group. Significantly different from the negative control group ().