The Effect of Traditional Chinese Formula Danchaiheji on the Differentiation of Regulatory Dendritic Cells
Effects of DCHJ on DC phenotype. ImDCs were prepared as described in Section 2. ImDCs and imDCs + LPS were stimulated with DCHJ (10% and 20%) for 48 h and then stained with FITC- or PE-conjugated antibodies against human CD86, CD11b, and HLA-DR. The corresponding isotype antibodies were used as negative controls. The expression of CD86, CD11b, and HLA-DR was detected by flow cytometry. (a) The numbers shown in the flow cytometry profiles are representative of positive cell percentages (%) and MFI. (b) The histograms represent quantification of the expression of the surface antigen of interest. Mean ± SD of data from three independent experiments is shown. , compared to the control group without LPS stimulation; ; , compared to the control group with LPS stimulation. DCHJ: Danchaiheji; MFI: mean fluorescence intensity.
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