Figure 3: Effects of DCHJ on DC-mediated antigen-specific CD4+ T cell proliferation. ImDCs with or without LPS pretreatment were cultured for 48 h in the presence or absence of DCHJ at various concentrations (10% and 20%). After 48 h incubation, cells were washed two times with RPMI 1640 medium. DCs were cocultured with T lymphocytes at a ratio of 1 : 10. After 5 days, cells were costained with anti-CD4-FITC and 7-AAD and counted by flow cytometry. The number of CD4+ 7-AAD cells represents the extent of CD4+ T cell proliferation. Mean ± SD of data from three independent experiments is shown. , compared to the control group without LPS stimulation; , compared to the control group with LPS stimulation. DCHJ; Danchaiheji; MFI: mean fluorescence intensity.