Figure 6: Mechanism of DCreg induction by DCHJ. ImDCs and imDCs + LPS were stimulated with DCHJ at concentrations of 20% for 48 h. Then, proteins and cDNA extracted from the coculture were prepared as described in Section 2. (a) The protein expression of IDO was detected by western blot. β-actin expression in each sample was used as a loading control. (b) The relative mRNA expression of IDO was determined by RT-PCR. Mean ± SD of data from three independent experiments is shown. ; , compared to the control group with LPS stimulation. DCHJ: Danchaiheji.