Evidence-Based Complementary and Alternative Medicine / 2016 / Article / Fig 5

Research Article

Inhibitory Effect of Valencene on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Figure 5

Effects of valencene on activation of the (a) STAT1 and (b) NF-κB signaling pathway in HaCaT cells. HaCaT cells were pretreated with VAL at the indicated concentration for 1 h and then exposed to TNF-α (10 ng/mL)/IFN-γ (10 ng/mL) for 30 min. Proteins were prepared and analyzed by western blot analysis using specific antibodies against p-IκB-α, IκB-α, NF-κB p65, STAT1, and p-STAT1. Effects of valencene on involucrin, loricrin, and filaggrin mRNA and protein expression. HaCaT keratinocytes were treated with valencene (10, 50, or 100 μM; 1 h) for 24 hr. (c) mRNA and (d) protein expression were determined by RT-PCR or western blotting, respectively. Data were expressed as the means ± SDs ( per experiment). versus TNF-α/IFN-γ alone or naïve control.
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