Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2016 (2016), Article ID 9404629, 9 pages
http://dx.doi.org/10.1155/2016/9404629
Research Article

Acupuncture Decreases NF-κB p65, miR-155, and miR-21 and Increases miR-146a Expression in Chronic Atrophic Gastritis Rats

1School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China
2Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China

Received 14 December 2015; Revised 4 April 2016; Accepted 13 April 2016

Academic Editor: Ching-Liang Hsieh

Copyright © 2016 Jialing Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Here are histology images in model group at various time points. As shown in Figure S1A, there is no obvious glandular atrophy at the end of week 8th. Relatively, at the end of week 12th (Figure S1B), histological observation showed that neutrophils and lymphocytes infiltration, cystic dilation, irregular arrangement and reduction of gastric glands were observed in rats with CAG, which met the diagnostic criteria. After 2 months withdraw of MNNG (Figure S1C), histological observation indicated that the characteristic changes (inflammatory infiltration, cystic dilation and reduction of gastric glands) of CAG still remained in model group. Consequently, it could be concluded that the CAG rat models induced by MNNG combined with irregular diet were stable. Figure S2 showed 2‒ΔCt values of miRNAs obtained from different groups. Expression levels of miR-155 and miR-21 were up-regulated significantly in model group than in control group and down-regulated significantly in acupuncture group than in model group, and there was no significance between acupuncture and control group. Relatively, expression level of miR-146a was down-regulated significantly in model group than in control group and up-regulated significantly in acupuncture group than in model group, and there was no significance between acupuncture and control group. Figure S3 showed that acupuncture may exerts its therapeutic effects via NF-κB-miR-155/miR-21/miR-146a signaling, which including (1) changes of transcription factors (such as NF-κB, which evoked by H. pylori infection, physical damage or chemical damage); (2) changes of miRNAs (miR-155/miR-21/miR-146a); (3) changes of downstream targets (such as TSLP, remain inconclusive).

  1. Supplementary Material