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Evidence-Based Complementary and Alternative Medicine
Volume 2016 (2016), Article ID 9406758, 8 pages
http://dx.doi.org/10.1155/2016/9406758
Research Article

Autophagy Protects from Raddeanin A-Induced Apoptosis in SGC-7901 Human Gastric Cancer Cells

1Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China
2Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China

Received 14 January 2016; Revised 15 March 2016; Accepted 23 March 2016

Academic Editor: Luciana Dini

Copyright © 2016 Yu-hao Teng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Raddeanin A (RA) is an extractive from Anemone raddeana Regel, a traditional Chinese medicine. The aim of this study is to assess the efficacy of RA against human gastric cancer (GC) cells (SGC-7901) and explore its mechanism. MTT assay showed that RA inhibition of proliferation of SGC-7901 cells increased in a dose-dependent manner. Flow cytometry analysis and Hoechst 33258 staining showed that RA induced apoptosis on SGC-7901 cells. Meanwhile, it induced autophagy. Western blotting analysis showed that the RA induces apoptosis and autophagy by activating p38 MAPK pathway and inhibiting mTOR pathway. Further studies showed that autophagy inhibition could protect from RA-induced apoptosis in SGC-7901 cells. In conclusion, RA can induce SGC-7901 cell apoptosis and autophagy by activating p38 MAPK pathway. And autophagy can protect SGC-7901 cells from apoptosis induced by RA.