Influence of the Melissa officinalis Leaf Extract on Long-Term Memory in Scopolamine Animal Model with Assessment of Mechanism of Action

Table 3

The effect of Melissa officinalis leaf extract on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities and AChE, BuAChE, or beta-secretase (BACE1) mRNA expression levels in frontal cortex (FC) or hippocampus (Hipp) of rats.

Group	Enzyme activity; [nmol/min/mg protein]				mRNA expression; [%]
	AChE		BuChE		ACHE		BuChE		BACE1
	FC	Hipp	FC	Hipp	FC	Hipp	FC	Hipp	FC	Hipp
MC + H2O	363 ± 49	439 ± 73	65 ± 11	53 ± 8	100 ± 12	100 ± 11	100 ± 18	100 ± 11	100 ± 16	100 ± 8
MO + H2O	276 ± 34	409 ± 28	69 ± 6	62 ± 4	48 ± 4	31 ± 7	16 ± 2	64 ± 21	36 ± 3	50 ± 8
HU + H2O	189 ± 15	239 ± 15	58 ± 6	51 ± 4	53 ± 13	85 ± 5	42 ± 9	102 ± 11	62 ± 6	98 ± 4
RA + H2O	224 ± 16	251 ± 12	77 ± 7	99 ± 7	101 ± 12	103 ± 5	184 ± 31	56 ± 7	126 ± 13	98 ± 3

Means ± SEM.
Number of animals: 7–10.
Values expressed as a ratio: the gene/GAPDH.
MC + H₂O: control group.
HU: huperzine A (0.5 mg/kg b.w., p.o.).
RA: rosmarinic acid (10 mg/kg b.w., p.o.).
Versus MC + H₂O, or , respectively.