Sakuranetin Inhibits Inflammatory Enzyme, Cytokine, and Costimulatory Molecule Expression in Macrophages through Modulation of JNK, p38, and STAT1

Figure 2

Effects of sakuranetin on the release of nitric oxide and the synthesis of inducible NO synthase (iNOS) and COX2 in LPS/interferon- (IFN-) γ stimulated cells. Mouse peritoneal macrophages were stimulated with IFN-γ and LPS in the presence of sakuranetin for 24 h. (a) NO in the supernatant was detected by the Griess reaction. Dexamethasone (1 μM) was treated as a reference drug. Data are expressed as mean ± SD (), and versus controls (LPS/IFN-γ treated cells). (b) The expression of iNOS and COX2 protein was analyzed by Western blotting using tubulin as an internal control. One of the three independent experiments is shown.

(a)

(b)