Research Article

Biatractylolide Modulates PI3K-Akt-GSK3β-Dependent Pathways to Protect against Glutamate-Induced Cell Damage in PC12 and SH-SY5Y Cells

Figure 8

Effects of biatractylolide on Akt and Gsk3β expressions of PC12 and SH-SY5Y cells induced by glutamate. (a)-(b) represent protein characterization of Akt and Gsk3β after PC12 and SH-SY5Y cells were treated with various concentrations of biatractylolide for 30 min before glutamate treatment for 24 hours. Blots were also probed for β-actin as loading controls. (c)-(d) showed the ratio of different proteins to β-actin was calculated by the band density of each cell line using Image J software. and versus control group; , , and versus model group.
(a) PC12
(b) SH-SY5Y
(c) PC12
(d) SH-SY5Y