Isolation and Characterization of Isofraxidin 7-O-(6′-O-p-Coumaroyl)-β-glucopyranoside from Artemisia capillaris Thunberg: A Novel, Nontoxic Hyperpigmentation Agent That Is Effective In Vivo

Figure 7

Effect of compound 1 on pigmentation in zebrafish embryos. (a) 9 hpf embryos were treated with PTU and two concentrations of isofraxidin or compound 1 (12.5 μM and 25 μM). Dorsal view of treated and untreated embryos at 72 hpf by stereomicroscope (magnification 100x). Isofraxidin and compound 1 increased melanocyte pigmentation. Scale bar = 250 μm. (b) Melanin content in zebrafish larvae treated with compound 1 or isofraxidin. 25 μM compound 1 significantly increased melanin content in the zebrafish larvae. (c) Tyrosinase activity in zebrafish larvae treated with compound 1 or isofraxidin. 12.5 μM or 25 μM compound 1 significantly increased tyrosinase activity in the zebrafish larvae. compared to the untreated control larvae.

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