Lectins, Interconnecting Proteins with Biotechnological/Pharmacological and Therapeutic Applications

Figure 5

Schematic representation of lectin histochemistry using peroxidase-conjugated lectins. In this hypothetical case, the lectin binds glycan moieties more expressed on normal tissues, which suffer a modification in their structure (e.g., sialylation or fucosylation) in benign and malign tumors tissues. Thus, the lectin binding increased in transformed tissues. The DAB reagent in the presence of peroxidase and hydrogen peroxide was converted to DAB oxidized that precipitates as a brown product and allows visualization of lectin binding.