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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 1796209, 9 pages
Research Article

Hepatoprotective Effect of Citral on Acetaminophen-Induced Liver Toxicity in Mice

1Department of Pharmacology and Therapeutics, State University of Maringá, Avenida Colombo 5790, 870020-900 Maringá, PR, Brazil
2Department of Chemistry, State University of Maringá, 87020-900 Maringá, PR, Brazil

Correspondence should be addressed to Nancy Sayuri Uchida; moc.liamtoh@iruyas.n and Roberto Kenji Nakamura Cuman; rb.meu@namucnkr

Received 9 March 2017; Revised 27 April 2017; Accepted 25 May 2017; Published 22 June 2017

Academic Editor: Yuri Clement

Copyright © 2017 Nancy Sayuri Uchida et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


High doses of acetaminophen (APAP) lead to acute liver damage. In this study, we evaluated the effects of citral in a murine model of hepatotoxicity induced by APAP. The liver function markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (γGT) were determined to evaluate the hepatoprotective effects of citral. The livers were used to determine myeloperoxidase (MPO) activity and nitric oxide (NO) production and in histological analysis. The effect of citral on leukocyte migration and antioxidant activity was evaluated in vitro. Citral pretreatment decreased significantly the levels of ALT, AST, ALP, and γGT, MPO activity, and NO production. The histopathological analysis showed an improvement of hepatic lesions in mice after citral pretreatment. Citral inhibited neutrophil migration and exhibited antioxidant activity. Our results suggest that citral protects the liver against liver toxicity induced by APAP.