Evaluation of an Aqueous Extract from Horseradish Root (Armoracia rusticana Radix) against Lipopolysaccharide-Induced Cellular Inflammation Reaction

Figure 3

Effect of horseradish root on MAPK pathway activation and TNF-α release. PBMC were stimulated with 1 μg/mL LPS for the indicated time points after treatment with the aqueous plant extract. (a, b) Total cell lysate was analyzed using immunoblotting. The picture shows representative blots of phosphorylated p-38 (Thr180/Tyr182) and ERK1/2 (Thr202/Tyr204) (a) or c-Jun (Ser73) (b). Blots were reprobed with antibodies against β-actin as loading control. (c) TNF-α was photometrically quantified in supernatants. The results were calculated relative to LPS stimulated cells; bars are mean ± SEM (). ; .

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