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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 2405124, 6 pages
https://doi.org/10.1155/2017/2405124
Research Article

Total Flavonoids Extracted from Oxytropis falcata Bunge Improve Insulin Resistance through Regulation on the IKKβ/NF-κB Inflammatory Pathway

1Central Laboratory, Gansu Province Academy of Chinese Medicine, Lanzhou, Gansu 730050, China
2Third Hospital Affiliated to Beijing University of Traditional Chinese Medicine, Beijing 100029, China
3School of Clinical Medicine, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu 730000, China
4Key Laboratory of Traditional Chinese Medicine of the Ministry of Education, Beijing University of Traditional Chinese Medicine, Beijing 100029, China

Correspondence should be addressed to Zhicheng Wang; moc.anis@611gnehcihzgnaw

Received 18 June 2016; Accepted 28 November 2016; Published 26 March 2017

Academic Editor: Dolores García Giménez

Copyright © 2017 Lixia Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Insulin resistance (IR) is the main etiology of type 2 diabetes mellitus (T2DM). It has been known that total flavonoid extracts can markedly improve the hypoglycemic symptoms caused by IR. Nevertheless, the relevant molecular mechanism remains unclarified. Aim. This study aimed to investigate the antihyperglycemic effects and mechanism of the total flavonoid extract from Oxytropis falcata Bunge. Methods. STZ-induced T2DM rats () were divided into 5 groups: model, low-, medium-, and high-dose total flavonoids, and pioglitazone groups. Ten healthy rats were used as controls. The serum insulin and inflammatory cytokines (MCP-1, TNF-α, and IL-6) level was measured by ELISA. The concentration of IRS-1, p-IRS-1, PKB p-PKB, PI3Kp85, and p-PI3K in skeletal muscles was determined by Western blot. The mRNA level of GLUT4, IκB, and NF-κB in skeletal muscle was detected by qRT-PCR. Results. The treatment of medium- and high-dose total flavonoids significantly reduced the FPG and P2hPG and enhanced insulin level in T2DM rats (). When compared with controls, the serum level of MCP-1, TNF-α, IL-6, IRS-1, and p-IRS-1 was significantly increased in T2DM rats, but the level of PKB, p-PKB, PI3Kp85, and p-PI3K expression was reduced (). The GLUT4 and IκB mRNA expression were significantly decreased, and NF-κB mRNA level was increased (). The treatment of low-, medium-, or high-dose total flavonoids markedly reversed the changes above (). Conclusion. Our study has confirmed the therapeutic effects of total flavonoids from Oxytropis falcata Bunge on IR. The flavonoids might reduce the production of inflammatory cytokines through downregulation of NF-κB expression in inflammatory pathway and regulate the IRS-1-PI3-K-PKB/Akt insulin pathway and thereby increased the GLUT4 expression.