Kidney-Replenishing Herb Induces SOCS-3 Expression via ERK/MAPK Pathway and Improves Growth of the First-Trimester Human Trophoblast Cells

Figure 4

SOCS-3 transcription in the Kidney-replenishing herb-treated human first-trimester trophoblast. As shown in (a), there were weak SOCS-3 transcription in group of vehicle control and no increased expression of SOCS-3 within 4 h. Then we further observed the transcription of SOCS-3 in the primary cultured first-trimester trophoblast cells after treatment with 10% (b) or 20% (c) Kidney-replenishing herb for different times. The slight SOCS-3 mRNA amount was determined in human trophoblasts response to treatment by 10% and 20% group of serum control at 2 h treatment ((d), (e)). We founded that SOCS-3 transcription increased slightly after Kidney-replenishing herb treatment for 1 h and reached its peak at 2 h, and Kidney-replenishing herb increased the transcription of SOCS-3 in trophoblast cells in a dose-dependent manner (f). , versus 20% control serum.