Orientin Ameliorates LPS-Induced Inflammatory Responses through the Inhibitory of the NF-κB Pathway and NLRP3 Inflammasome

Figure 4

Effects of orientin treatment on LPS-induced the NF-κB activation in RAW 264.7 cells. Cells were pretreated with Ori (10 or 40 μM) for 6 h and then were exposed to LPS for 30 min. The protein levels of IκBα and P-IκBα as well as the nuclear and cytoplasmic levels of NF-κB (p65) were analyzed by Western blot. The relative densities of protein were performed by densitometric analysis; β-actin and Lamin B were used acted as an internal control, respectively. Similar data were repeated in three independent experiments, and one of three representative experiments is shown. All data from three independent experiments are presented as means ± SEM. versus the control group; and versus the LPS group.

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