Evidence-Based Complementary and Alternative Medicine / 2017 / Article / Fig 2

Research Article

Icariin Prevents H2O2-Induced Apoptosis via the PI3K/Akt Pathway in Rat Nucleus Pulposus Intervertebral Disc Cells

Figure 2

The sectionalization: (A) blank control. (B) 200 μM H2O2. (C) 200 μM H2O2 + 20 μM icariin (Ica). (D) 200 μM H2O2 + 20 μM Ica. (E) 200 μM H2O2 + 20 μM Ica + 25 μM LY294002. (a) Phase-contrast light microscopy observations: the cell number of group (B) is decreased, cells are more shrunk, and there were many round dead cells. In group (C), there were fewer dead cells and the cell morphology was more normal than in group (B). However, in group (D), cells shrank and there were many dead cells. (b) Apoptosis detected by flow cytometry and fluorescence microscope: the extent of apoptosis significantly declined in group (C) compared with group (B). In group (D), the extent of apoptosis increased compared with group (C). Group (E) exhibited the most apoptotic cells of all groups. (c) Apoptosis rate: the apoptosis rate of H2O2 group increased significantly and the cytotoxicity of H2O2 is weakened by icariin ( versus control group; versus H2O2 group). In addition, the lock of PI3/AKT pathway made a dent in the protection effect of icariin ( versus group (C)). Group (E) (25 μM LY294002 + 200 μM H2O2): this group exhibited more apoptotic cells ( versus group (D)).

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