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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 2906014, 8 pages
Research Article

Preventive Effect and Safety of a Follicle Stimulating Hormone Inhibitory Formulation Containing a Mixture of Coicis Semen and Artemisia capillaris for Precocious Puberty: A Preliminary Experimental Study Using Female Rats

1College of Korean Medicine, Gachon University, Seongnam, Republic of Korea
2Highki Korea Medicine Clinic, 6 Dong 5F, 205, Shinbanpo-ro, Seocho-gu, Seoul, Republic of Korea
3East West Cancer Center, Dunsan Korean Medicine Hospital of Daejeon University, Daejeon, Republic of Korea

Correspondence should be addressed to Hwa Seung Yoo; rk.ujd@shytla and Hye Lim Lee;

Received 2 August 2017; Revised 29 September 2017; Accepted 16 October 2017; Published 20 November 2017

Academic Editor: Darren R. Williams

Copyright © 2017 Tuy An Trinh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Precocious puberty is a common endocrine disease in children. Inappropriate activation of hypothalamic–pituitary–gonadal axis leads to the development of secondary sexual characteristics at an earlier age than normal children and causes short stature in adulthood. Objectives. The aim of this study is to evaluate the preventive effects of a herbal formulation containing a mixture of Coicis Semen and Artemisia capillaris (hEIF extract) on precocious puberty. Methods. The preventive effect of hEIF extract on precocious puberty in rats was evaluated by measuring blood component after 3 weeks of treatment via oral administration. Network pharmacological analyses were performed to predict the bioactive components of hEIF extract. Results. In vivo studies showed that hEIF extract significantly reduced follicle stimulating hormone (FSH) levels. After treatment with 200 mg/kg of hEIF extract, the FSH level was  ng/mL, whereas the FSH level in the vehicle group was  ng/mL. Moreover, the use of hEIF extract did not stimulate body growth and bone accretion in rats. The network pharmacological analysis led to the identification of multiple targets of hEIF extract related to lipolysis and the female sex hormone-related pathways. Conclusion. hEIF extract can be used as an FSH inhibitor for the treatment of precocious puberty.