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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 2915247, 8 pages
https://doi.org/10.1155/2017/2915247
Research Article

The Effects of Guizhi Gancao Decoction on Pressure Overload-Induced Heart Failure and Posttranslational Modifications of Tubulin in Mice

1Department of Pathology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2Public Experiment Platform, School of Basic Medical Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
3Department of Physiology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

Correspondence should be addressed to Chen Zhang and Rong Lu

Received 11 February 2017; Accepted 13 June 2017; Published 17 July 2017

Academic Editor: Settimio Grimaldi

Copyright © 2017 Hui-hua Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Guizhi Gancao Decoction (GGD), a traditional Chinese medical recipe, has been widely used in the treatment of cardiovascular diseases in China for centuries. The present study was carried out to determine whether GGD exerts direct protective effects against pressure overload-induced heart failure. Moreover, we investigated whether GGD affects tubulin expression and posttranslational modifications. We demonstrated that GGD ameliorated TAC caused cardiac hypertrophy by gravimetric and echocardiography analysis in C57BL/6 mice. We found that GGD abrogated TAC-induced myocardium fibrosis by Masson’s staining and collagen volume fraction (CVF) analysis. By using pressure-volume hemodynamic measurements, we found that GGD prevented TAC-induced cardiac systolic and diastolic dysfunction. Immunoblotting and immunofluorescent analysis revealed that GGD abrogated TAC-induced detyrosination and acetylation abnormalities on microtubules. Our present study demonstrated potential therapeutic effects of GGD against pressure overload-induced heart failure.