Compound Wumei Powder Inhibits the Invasion and Metastasis of Gastric Cancer via Cox-2/PGE2-PI3K/AKT/GSK3β/β-Catenin Signaling Pathway

Figure 3

CWP inhibited the expression of Cox-2, MMP-7, and PGE2. (a) Extracts from transplanted tumor were analyzed for the expression of Cox-2. (b) The histogram shows the expression of Cox-2 compared to the control group in vivo. (c) Extracts from SGC-7901 cells were analyzed for the expression of Cox-2. (d) The histogram shows the expression of Cox-2 compared to the control group in vitro. (e) Immunohistochemistry assay was used to detect the expression of Cox-2 of transplanted tumor; expression levels are higher in control group. (f) The histogram shows the mean density (average density) of Cox-2. (g) The histogram shows the mRNA expression of Cox-2 of the tumor by RT-PCR in vivo. (h) The histogram shows the mRNA expression of Cox-2 of the tumor by RT-PCR in vitro. (i) The histogram shows the level of PGE2 in nude mice by ELISA method. (j) The images show melt curve and amplification plot of (g). (k) The images show melt curve and amplification plot of (h) ((I) and (II) show the melt curve of β-actin and Cox-2; (III) and (IV) show the amplification plot of β-actin and Cox-2). Data are expressed as the mean ± SD of three experiments ( < 0.01 compared with control group).

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