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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 3526392, 15 pages
https://doi.org/10.1155/2017/3526392
Research Article

Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts

1Department of Physico-Chemistry of Microorganisms, Institute of Genetics and Microbiology, Wroclaw University, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland
2Faculty of Biotechnology, Wroclaw University, Ul. Przybyszewskiego 63/77, 51-148 Wroclaw, Poland
3Department of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant Sciences, Wroclaw, Poland

Correspondence should be addressed to Magdalena Czemplik; lp.ude.rwu@zcabuh-kilpmezc.aneladgam

Received 24 April 2017; Revised 18 July 2017; Accepted 5 September 2017; Published 24 October 2017

Academic Editor: Daniela Rigano

Copyright © 2017 Magdalena Czemplik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The goal of this study was to evaluate the most effective technique for extraction of phenolics present in flax shives and to assess their effect on human fibroblasts. Flax shives are by-products of fibre separation, but they were found to be a rich source of phenolic compounds and thus might have application potential. It was found that the optimal procedure for extraction of phenolics was hydrolysis enhanced by the ultrasound with NaOH for 24 h at 65°C and subsequent extraction with ethyl acetate. The influence of the flax shives extract on fibroblast growth and viability was assessed using the MTT and SRB tests. Moreover, the influence of flax shives extract on the extracellular matrix remodelling process was verified. The 20% increase of the viability was observed upon flax shives extract treatment and the decrease of mRNA collagen genes, an increase of matrix metalloproteinase gene expression, and reduction in levels of interleukin 6, interleukin 10, and suppressor of cytokinin signaling 1 mRNA were observed. Alterations in MCP-1 mRNA levels were dependent on flax shives extract concentration. Thus, we suggested the possible application of flax shives extract in the wound healing process.