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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 3526392, 15 pages
https://doi.org/10.1155/2017/3526392
Research Article

Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts

1Department of Physico-Chemistry of Microorganisms, Institute of Genetics and Microbiology, Wroclaw University, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland
2Faculty of Biotechnology, Wroclaw University, Ul. Przybyszewskiego 63/77, 51-148 Wroclaw, Poland
3Department of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant Sciences, Wroclaw, Poland

Correspondence should be addressed to Magdalena Czemplik

Received 24 April 2017; Revised 18 July 2017; Accepted 5 September 2017; Published 24 October 2017

Academic Editor: Daniela Rigano

Copyright © 2017 Magdalena Czemplik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table 1: Identification and quantification of phenolic compounds in the M50 flax shives hydrolysed extract (A) and the vanillin content depending on the used extraction solvent (B).

Figure 1: Total phenolic content of flax shives extracts depending on the used extraction solvent. For the determination of statistical significance ANOVA test was used (** - P < 0.01, *** - P < 0.001).

Figure 2: The effect of flax shives extract on NHDF cells in in vitro SRB test after 24h (A) and 48h (B) of incubation. The numbers 1-6 correspond to the NHDF cells treated with shives extracts #1-#6. The analyses were performed in three biological replicates. For the determination of statistical significance For the determination of statistical significance ANOVA test was used.

  1. Supplementary Material