Apigenin Inhibits Human SW620 Cell Growth by Targeting Polyamine Catabolism

Figure 6

(a) Analysis of putrescine, spermidine, spermine, and total polyamine contents (nmol/mg protein) in SW620 cells treated with apigenin (10, 20, and 40 μM), exogenous-spermidine (5 μM), DFMO (2.5 mM), and a combination of DFMO and apigenin (10, 20, and 40 μM) for 48 h. Total polyamine contents were decreased by both apigenin and DFMO. Furthermore, the combination of apigenin and DFMO induced an even greater decrease in polyamine levels. and versus control. (b) Cells exposed to increased concentrations of apigenin demonstrate a concentration-dependent increase in putrescine level and decrease in spermine level with a decrease in total polyamine content. Accordingly, intergroup and intragroup polyamine levels were analyzed. and compare doses; and versus control. (c) In order to investigate the effect of a combination of DFMO and apigenin on polyamine levels, increasing concentrations of apigenin were combined with DFMO. Total polyamine content was analyzed by intergroup comparison. and compare doses; and versus control.

(a)

(b)