Apigenin Inhibits Human SW620 Cell Growth by Targeting Polyamine Catabolism

Table 2

Polyamine content (nmol/mg protein) of SW620 cells after exposure to different concentrations of apigenin (10, 20, and 40 μM), DFMO (2.5 mM), and a combination of DFMO and apigenin for 48 h. The effect of exogenous-spermidine (5 μM) after 48 h of treatment is also shown. The value was determined by one-way analysis with Dunnett’s posttest. and versus control.

Treatment	Growth parameter (nmol/mg protein)
	Putrescine	Spermidine	Spermine	Total polyamines
Ctrl	3.01 ± 0.05	12.53 ± 0.02	18.15 ± 0.07	33.69 ± 0.02
DFMO	1.66 ± 0.12	8.12 ± 0.82	13.78 ± 0.29	23.55 ± 0.97
SPD	7.06 ± 0.23	28.37 ± 0.65	24.88 ± 0.41	60.37 ± 1.17
10 μM Api	4.44 ± 0.46	13.07 ± 0.52	12.64 ± 0.56	30.15 ± 0.96
20 μM Api	5.81 ± 0.44	11.53 ± 0.42	9.77 ± 0.36	27.12 ± 0.58
40 μM Api	7.52 ± 0.27	6.39 ± 0.43	7.04 ± 0.03	20.86 ± 0.26
DFMO + 10 μM Api	3.40 ± 0.28	8.47 ± 0.60	11.53 ± 0.42	23.40 ± 1.14
DFMO + 20 μM Api	2.66 ± 0.18	6.39 ± 0.44	7.39 ± 0.61	16.44 ± 0.88
DFMO + 40 μM Api	1.46 ± 0.17	4.31 ± 0.21	5.50 ± 0.33	11.27 ± 0.50