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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 3729547, 7 pages
https://doi.org/10.1155/2017/3729547
Research Article

Therapeutic Effect and Mechanism of Oxytropis falcata Gel on Deep Second-Degree Burn in Rats

1Department of Pharmacy, The 175th Hospital of PLA, Affiliated Southeast Hospital of Xiamen University, Fujian, Zhangzhou 363000, China
2Department of Pathology, The 175th Hospital of PLA, Affiliated Southeast Hospital of Xiamen University, Fujian, Zhangzhou 363000, China

Correspondence should be addressed to Jin-Shan Chen and Yan Fei

Received 20 June 2017; Accepted 4 October 2017; Published 13 November 2017

Academic Editor: Caio P. Fernandes

Copyright © 2017 Xiao-Feng Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Oxytropis falcata has long been used to treat inflammation, sores, and bleeding in Tibet. However, the burn remedy and underlying molecular mechanisms are not well understood. This study is aimed at assessing the effect of Oxytropis falcate gel (OFG) on deep second-degree burn rats and exploring its mechanism. Wistar rats with second-degree burn were treated with OFG and silver sulfadiazine. Immunohistochemical detections for EGF and VEGF were performed, and ELISA detections for EGF, VEGF, p38, and IL-1β in serum were determined. Rats treated with OFG (25, 50 g/kg) consisted of the major rhamnocitrin-3-O-β-neohesperidoside significantly accelerated incrustation () and decrustation (). According to HE staining, edema and infiltration of inflammatory cells decrease apparently with good hyperplasia and incrustation in administration groups (7 d). The expressions of EGF and CD34 in OFG (25, 50 g/kg) treatment increased obviously from immunohistochemical assessment (7 d). Serum EGF expression reached  ng/mL by OFG treatment, while p38 () and IL-1β () levels were significantly lower than the model and vehicle groups from day 1 to day 7. OFG possesses potential wound healing activities. The mechanism may be related to the increasing of biosynthesis and the releasing of EGF and CD34 and the decreasing p38 and IL-1β levels.