Effect of QSKL on MAPK and RhoA Pathways in a Rat Model of Heart Failure

Table 1

Nucleotide sequences of primers used in real-time PCR.

Gene	Primers	Nucleotide sequences 5′-3′	Length (bp)	Temp. (°C)
ERK	Forward	AAAAGGGGGAGGGGACGTAA	87	60.5
	Reverse	CCACTCACCTGTTTCCTGTCT		59.6
JNK	Forward	GCCTGGACTGTGACGTCTATTT	195	60.2
	Reverse	AGCATTCCCTACAAAGTGCC		58.7
RhoA	Forward	TGTTTTTCCATCGACAGCCCT	171	60.2
	Reverse	TACCGGCTCCTGCTTCATTT		59.4
ROCK1	Forward	ACCTTCTGGCTTTGTCCGT	159	59.2
	Reverse	CCTGGTGATCAGGTAGCAT		57.4
ROCK2	Forward	TCCCTTCCCTGTGGTGACTT	88	60.4
	Reverse	GGGAAAGGAGCTGGTTCAAGA		59.9
MLC	Forward	TCGACGCCATGATGAAGGAA	200	59.5
	Reverse	CTGGGAAAATCGGTCGCACT		60.7
GAPDH	Forward	ATTCCATCCCAGACCCCATAAC	81	59.5
	Reverse	GCAGCGAACTTTATTGATGGTAT		57.6