TY - JOUR A2 - Lee, Bonghyo AU - Jeon, Hyongjun AU - Ryu, Sun AU - Kim, Dongsoo AU - Koo, Sungtae AU - Ha, Ki-Tae AU - Kim, Seungtae PY - 2017 DA - 2017/05/16 TI - Acupuncture Stimulation at GB34 Restores MPTP-Induced Neurogenesis Impairment in the Subventricular Zone of Mice SP - 3971675 VL - 2017 AB - Adult neurogenesis has recently been considered a new therapeutic paradigm of Parkinson’s disease. In this study, we investigated whether acupuncture restores 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine- (MPTP-) induced impaired neurogenesis in the subventricular zone (SVZ). Male C57BL/6 mice were given 30 mg/kg of MPTP intraperitoneally once a day for 5 days, after which they were intraperitoneally injected with 50 mg/kg of bromodeoxyuridine (BrdU) and given acupuncture stimulation at HT7 or GB34 for 12 consecutive days. Dopaminergic neuronal survival in the nigrostriatal pathway and cell proliferation in the SVZ was then evaluated by immunostaining. MPTP administration induced dopaminergic neuronal death in the nigrostriatal pathway, which was suppressed by acupuncture stimulation at GB34. MPTP administration also suppressed the number of BrdU-positive cells and glial fibrillary acidic protein/BrdU-positive cells and increased the number of doublecortin/BrdU-positive cells in the SVZ, which were restored by acupuncture stimulation at GB34. These results indicate that acupuncture stimulation at GB34 restores MPTP-induced neurogenesis impairment. SN - 1741-427X UR - https://doi.org/10.1155/2017/3971675 DO - 10.1155/2017/3971675 JF - Evidence-Based Complementary and Alternative Medicine PB - Hindawi KW - ER -