1H NMR-Based Metabonomic Study of Functional Dyspepsia in Stressed Rats Treated with Chinese Medicine Weikangning
Table 1
Quantitative comparison of metabolites identified in urine of model group (MS) and normal control group (NS).
Metabolites
Chemical shift
Integral in MS group (mean ± S.D.) × 10−2
Integral in NS group (mean ± S.D.) × 10−2
VIP
Leucine
0.95 (t)
10.112 ± 1.155
8.732 ± 1.004
(↑)
1.779
0.004
Lactate
1.335 (d)
8.332 ± 0.997
7.284 ± 0.951
(↑)
1.845
0.011
Alanine
1.485 (d)
6.328 ± 0.539
5.582 ± 0.614
(↑)
2.135
0.002
Acetate
1.93 (s)
7.851 ± 2.285
14.826 ± 27.119
(↓)
1.735
0.031
N-Acetylglutamate
2.225 (t)
10.518 ± 0.978
12.481 ± 1.975
(↓)
1.981
0.013
Succinate
2.41 (s)
16.494 ± 5.225
27.520 ± 12.593
(↓)
1.996
0.023
2-Oxoglutarate
2.46 (t), 3.015 (t)
48.701 ± 16.029
72.679 ± 25.975
(↓)
1.966
0.005
Citrate
2.565 (AB)
21.411 ± 40.034
73.047 ± 45.443
(↓)
2.166
0.004
Dimethylamine
2.725 (s)
64.191 ± 12.555
48.890 ± 9.897
(↑)
1.951
0.002
Trimethylamine
2.92 (s)
3.671 ± 0.718
4.701 ± 0.948
(↓)
1.753
0.003
Phenylacetylglycine
3.68 (s)
34.812 ± 6.582
27.550 ± 3.781
(↑)
1.794
0.003
Hippurate
3.965 (t), 7.71 (d), 7.83 (d)
17.012 ± 2.050
14.239 ± 1.772
(↑)
1.897
0.001
Allantoin
5.39 (s)
117.921 ± 32.112
88.265 ± 30.123
(↑)
1.623
0.035
Cytidine
6.07 (d)
11.707 ± 2.572
7.857 ± 1.949
(↑)
1.968
0.000
Tyrosine
7.28 (s)
7.227 ± 1.931
5.411 ± 1.349
(↑)
1.618
0.009
Imidazole
7.365 (t)
25.161 ± 7.959
15.153 ± 3.917
(↑)
1.888
0.001
Phenylalanine
7.425 (t)
9.045 ± 2.891
5.604 ± 1.378
(↑)
1.782
0.001
The relative integrals of metabolites were determined by 1D 1H NMR analysis of urine. The values of the correlation number extracted from the correlation plots of OPLS-DA models of MS versus NS. The P values were obtained from Independent-Samples t-test or -test (shown with ). The P values were calculated by Mann–Whitney U test of nonparametric tests and the related integrals were presented in median ± IQR The arrows (↑/↓) denote higher/lower amounts relative to NS group.
