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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 4287890, 10 pages
Research Article

Resveratrol Ameliorates Experimental Alcoholic Liver Disease by Modulating Oxidative Stress

1Department of Gastroenterology, The Affiliated Hospital of Chengde Medical University, Chengde, Hebei 067000, China
2Department of Pathology, Chengde Medical University, Chengde, Hebei 067000, China
3Precision Nutrition Innovation Center, School of Public Health, Zhengzhou University, Zhengzhou 450001, China

Correspondence should be addressed to Hou Zhiping; moc.361@uohgnipihz and Mustapha Umar Imam; moc.liamg@mamiytsum

Received 30 June 2017; Revised 8 November 2017; Accepted 28 November 2017; Published 31 December 2017

Academic Editor: Eman Al-Sayed

Copyright © 2017 He Peiyuan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to investigate the hepatoprotective effects of resveratrol in alcoholic liver disease (ALD). Alcohol was administered to healthy female rats starting from 6% (v/v) and gradually increased to 20% (v/v) by the fifth week. After 16 weeks of intervention, liver enzymes (aspartate aminotransferase [AST] and alanine aminotransferase [ALT]) were analyzed using a chemistry analyzer, while hepatic antioxidant enzymes, oxidative stress markers, and caspase 3 activity were assessed using ELISA kits. Furthermore, hepatic CYP2E1 protein levels and mRNA levels of antioxidant and inflammation-related genes were determined using western blotting and RT-PCR, respectively. The results showed that resveratrol significantly attenuated alcohol-induced elevation of liver enzymes and improved hepatic antioxidant enzymes. Resveratrol also attenuated alcohol-induced CYP2E1 increase, oxidative stress, and apoptosis (caspase 3 activity). Moreover, genes associated with oxidative stress and inflammation were regulated by resveratrol supplementation. Taken together, the results suggested that resveratrol alleviated ALD through regulation of oxidative stress, apoptosis, and inflammation, which was mediated at the transcriptional level. The data suggests that resveratrol is a promising natural therapeutic agent against chronic ALD.