Resveratrol Ameliorates Experimental Alcoholic Liver Disease by Modulating Oxidative Stress
Table 2
Names, accession number, and primer sequences used in the study.
Gene name
Accession number
Forward sequence
Reverse sequence
Kan(r)
Beta-actin
NM_031144
AGGTGACACTATAGAATA GGCATCCTGACCCTGAAGTA
GTACGACTCACTATAGGGA AGACGCAGGATGGCATGAG
SOD1
NM_017050
AGGTGACACTATAGAATA TCAATATGGGGACAATACAC
GTACGACTCACTATAGGGA TACTTTCTTCATTTCCACCTT
SOD2
NM_017051
AGGTGACACTATAGAATA TGTATGAAAGTGCTCAAGAT
GTACGACTCACTATAGGGA GCCCTCTTGTGAGTATAAGT
SOD3
NM_012880
AGGTGACACTATAGAATA TCGAACTACTTTATGCCC
GTACGACTCACTATAGGGA GAAGACAAACGAGGTCTCTA
CAT
NM_012520
AGGTGACACTATAGAATA ACTGCAAGTTCCATTACAAG
GTACGACTCACTATAGGGA GTTCAACTTCAGCAAAATAAT
GPx
NM_001039848
AGGTGACACTATAGAATA GTGCCAGAGCCGGGG
GTACGACTCACTATAGGGA GGTCTTGCCTCACTGGGA
CYP2E1
NM_031543
AGGTGACACTATAGAATA AGAGGGAATTGGATC
GTACGACTCACTATAGGGA TGCCTGGGATCAGGTTA
FasL
NM_010177
AGGTGACACTATAGAATA GTGCCCCGGAATTGGG
GTACGACTCACTATAGGGA GGGCTCAATTCCCAA
TNF-α
NM_001024447
AGGTGACACTATAGAATA GCCAAGGGGTTGGGAA
GTACGACTCACTATAGGGA GGGACTTTGGGGGAAA
Housekeeping gene. Underlined sequences are left and right universal left and right sequences (tags). Internal control supplied by Beckman Coulter Inc. (Miami, FL, USA) as part of the GeXP kit. RT conditions were 48°C for 1 min; 37°C for 5 min; 42°C for 60 min; 95°C for 5 min and then held at 4°C. PCR conditions were initial denaturation at 95°C for 10 min, followed by two-step cycles of 94°C for 30 sec and 55°C for 30 sec, ending in a single extension cycle of 68°C for 1 min. IDE: insulin degrading enzyme; LRP: low density lipoprotein receptor-related protein 1; SOD: superoxide dismutase; CAT, catalase; PSEN: presenilin; APP: amyloid precursor protein. Gapdh: glyceraldehyde-3-phosphate dehydrogenase; Kan(r): kanamycin resistance.
