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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 4389048, 10 pages
https://doi.org/10.1155/2017/4389048
Research Article

Dai-Huang-Fu-Zi-Tang Alleviates Intestinal Injury Associated with Severe Acute Pancreatitis by Regulating Mitochondrial Permeability Transition Pore of Intestinal Mucosa Epithelial Cells

1Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian 116001, China
2Gastrointestinal Surgery, Liaocheng City People’s Hospital, Liaocheng 252000, China
3Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China
4Graduate School, Dalian Medical University, Dalian 116044, China
5Graduate Schools, Zunyi Medical College, Zunyi 563003, China

Correspondence should be addressed to Xiaoguang Lu

Received 18 July 2017; Accepted 21 November 2017; Published 18 December 2017

Academic Editor: Omer Kucuk

Copyright © 2017 Xin Kang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The aim of the present study was to examine whether Dai-Huang-Fu-Zi-Tang (DHFZT) could regulate mitochondrial permeability transition pore (MPTP) of intestinal mucosa epithelial cells for alleviating intestinal injury associated with severe acute pancreatitis (SAP). Methods. A total of 72 Sprague-Dawley rats were randomly divided into 3 groups (sham group, SAP group, and DHFZT group, per group). The rats in each group were divided into 4 subgroups ( per subgroup) accordingly at 1, 3, 6, and 12 h after the operation. The contents of serum amylase, D-lactic acid, diamine oxidase activity, and degree of MPTP were measured by dry chemical method and enzyme-linked immunosorbent assay. The change of mitochondria of intestinal epithelial cells was observed by transmission electron microscopy. Results. The present study showed that DHFZT inhibited the openness of MPTP at 3, 6, and 12 h after the operation. Meanwhile, it reduced the contents of serum D-lactic acid and activity of diamine oxidase activity and also drastically relieved histopathological manifestations and epithelial cells injury of intestine. Conclusion. DHFZT alleviates intestinal injury associated SAP via reducing the openness of MPTP. In addition, DHFZT could also decrease the content of serum diamine oxidase activity and D-lactic acid after SAP.