Anti-Inflammatory Effect of Piper attenuatum Methanol Extract in LPS-Stimulated Inflammatory Responses

Figure 3

Effect of Pa-ME on the activation of upstream signaling molecules for NF-κB translocation. (a and b) Phosphorylated or total forms of IκBα, IKKα/β, AKT, Src, Syk, and β-actin levels at 5, 15, 30, and 60 min or 2, 3, and 5 min were detected by immunoblotting analysis with phospho-specific or total protein antibodies from total cell lysates. (c) The binding level of p-p85 to Src (left panel) or Syk (right panel) at 2 min was detected by immunoprecipitation and immunoblotting analysis of LPS-treated RAW264.7 cells in the presence or absence of Pa-ME.

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