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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 4673289, 10 pages
Research Article

The Proliferation Enhancing Effects of Salidroside on Schwann Cells In Vitro

Hui Liu,1,2,3 Peizhen Lv,1,4 Huayu Wu,5 Kun Zhang,1,2 Fuben Xu,6,7 Li Zheng,1,3 and Jinmin Zhao1,2,3

1Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, China
2Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China
3Guangxi Collaborative Innovation Center for Biomedicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, China
4Department of Spine Surgery, The Third Affiliated Hospital of Guangxi Medical University, Nanning, China
5Department of Cell Biology & Genetics, School of Premedical Sciences, Guangxi Medical University, Nanning, China
6Department of Genetic Metabolism, Children’s Hospital, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning 530003, China
7Guangxi Center for Birth Defects Research and Prevention, Nanning 530003, China

Correspondence should be addressed to Li Zheng; nc.ude.umxg@ilgnehz and Jinmin Zhao; moc.621@nimnijoahz

Received 7 November 2016; Revised 2 April 2017; Accepted 10 April 2017; Published 7 June 2017

Academic Editor: G. K. Jayaprakasha

Copyright © 2017 Hui Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Derived from Rhodiola rosea L., which is a popular plant in Eastern Europe and Asia, salidroside has pharmacological properties including antiviral, anticancer, hepatoprotective, antidiabetic, and antioxidative effects. Recent studies show that salidroside has neurotrophic and neuroprotective effects. However, the effect of salidroside on Schwann cells (SCs) and the underlying mechanisms of the salidroside-induced neurotrophin secretion have seldom been studied. In this study, the effect of salidroside on the survival, proliferation, and gene expression of Schwann cells lineage (RSC96) was studied through the examinations of the cell viability, proliferation, morphology, and expression of neurotrophic factor related genes including BDNF, GDNF, and CDNF at 2, 4, and 6 days, respectively. These results showed that salidroside significantly enhanced survival and proliferation of SCs. The underlying mechanism might involve that salidroside affected SCs growth through the modulation of several neurotrophic factors including BDNF, GDNF, and CDNF. As for the concentration, 0.4 mM, 0.2 mM, and 0.1 mM of salidroside were recommended, especially 0.2 mM. This investigation indicates that salidroside is capable of enhancing SCs survival and function in vitro, which highlights the possibility that salidroside as a drug agent to promote nerve regeneration in cellular nerve scaffold through salidroside-induced neurotrophin secretion in SCs.